Jan 11, 2016 for a human, a genomic reference sequence does not contain any useful information (a coding DNA reference sequence does); a gene can be

2000-09-01

NRSE motifs associated with protein-coding genes were located ≤ 100 kb distance. The NRSEs in GD were located > 100 kb from a protein-coding gene. Additional file 3: Table S3. (43K, xlsx) canonical RE1/NRSE sites, which have a variable linker between the two half-site RE1s/NRSEs,7 while a third type features half-site RE1/NRSE with only one-half of the canonical RE1/NRSE.7 Of note, variations of the RE1/NRSE sequence are associated with modulation of REST/NRSF affinity to the respective DNA site, allowing some RE1/NRSE sites 2006-09-26 Plasmid pAAV-DR1-NRSE-RFP from Dr. Edward Callaway's lab contains the insert peroxisome proliferator activated receptor gamma binding site promoter with neuron-restrictive silencer element with minimal CMV and is published in Front Neural Circuits. 2009 .

2015-02-11 · The neuron-restrictive silencer factor (NRSF) binds with the neuron-restrictive silencer element (NRSE), thereby suppressing the transcription of NRSE-containing genes. In this study, we show that the NRSE sequence of the VGF gene critically regulates the repression of VGF expression in NMB cells. (A) The putative Sp family binding site and NRSE sequence among three species, mouse, human and rat is located from −9 to +20 in the MOR gene and have highly homologous (∗) sequence to the consensus Sp family binding site and NRSE DNA element. NRSE engineered superior cervical ganglion (SCG)10 promoter activities.

Consensus NRSEs have been identified in 18 neuron-specific genes. Complementary DNA clones encoding a functional fragment of NRSF were isolated and found to encode a novel protein containing eight We generated oligodeoxynucleotides (ODNs) comprised of the NRSF binding sequence (NRSE), modified their backbone for stability and infused them into the brain.

NRSE is a regulatory sequence that is present in several neuronal genes (8) and that was, up to now, thought to silence neuronal gene transcription in nonneuronal cells (6, 7, 9 – 14).

We report here that this KCC2 element binds to nuclear proteins and inhibits the transcription of a reporter gene in C17 nonneuronal cells. METHODS Genomic library A Lamda FIXII mouse genomic library (Stratagene, La Jolla, CA) The identification of a common cis -acting silencer element, a neuron-restrictive silencer element (NRSE), in multiple neuron-specific genes, together with the finding that zinc finger transcription factor REST/NRSF/XBR could confer NRSE-mediated silencing in non-neuronal cells, suggested that REST/NRSF/XBR is a master negative regulator of neurogenesis. NRSE sequence in the HCN1 gene and the structure of the NRSF gene are conserved in humans, mice, and rats [8], as shown in Figure 1.

NRSE-like sequences are found in intron 8 and exon 9 of the human CYP11B2 and CYP11B1 genes. There are no NRSE-like sequences in the rat CYP11B2 and CYP11B1 genes. Moreover, the human and rat CACNA1H genes, which encode the α-subunit of Cav3.2, contain a functional NRSE sequence .

8,18,19,47 Next, we investigated the expression of NRSF in the ipsilateral spinal dorsal horn or L2-4 DRG after sarcoma cell inoculation at both transcriptional and translational levels. NRSE-like sequences are present in the human CYP11B2 (hCYP11B2) and CYP11B1 (hCYP11B1) genes.

Hence, the regulatory activity of both NRSE and NRSF in mammals prevents not only neural dysfunctions, but also physiological and phenotypical abnormalities in other non-neuronal regions of the body. 2007-06-08 The only likely repressor sequence was NRSE, which interacts with the silencer NRSF. NRSF, a zinc finger transcription factor that binds to the 21-bp recognition sequence NRSE, is a transcriptional repressor of multiple neuronal genes and is normally down-regulated upon neuronal differentiation (44. Su X. To address this, we investigated the effect of REST/NRSF and REST4 on the activity-dependent activation of BDNF gene promoter I (BDNFp-I) using cultured rat cortical neurons. REST/NRSF markedly repressed the transcriptional activation of BDNFp-I, whereas the effect of REST4 was weak, depending upon the NRSE/RE1 sequence.
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There are no NRSE-like sequences in the rat CYP11B2 and CYP11B1 genes. Moreover, the human and rat CACNA1H genes, which encode the α-subunit of Cav3.2, contain a functional NRSE sequence . To better restrict transgene expression to the nervous system in zebrafish larvae, we have used DNA sequences derived from the neuron-restrictive silencing element (NRSE). We find that one such sequence, REx2, when used in conjunction with several basal promoters, robustly suppresses transgene expression in non-neuronal tissues. 2006-04-20 The silencer activity of this region is mediated solely by a repressor element 1 or neuron-restrictive silencer element (RE1/NRSE).

As shown in Fig. 4B, each reporter containing the intact NRSE sequence (left panel) exhibited less luciferase activity than those with the mutated NRSE sequences (right panel).
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In addition to the influence on the ANP gene, the NRSE sequence regulates other cardiac embryonic genes, such as BNP, skeletal α-actin, and Na, K – ATPase α3 subunit. Hence, the regulatory activity of both NRSE and NRSF in mammals prevents not only neural dysfunctions, but also physiological and phenotypical abnormalities in other non-neuronal regions of the body.

As shown in Fig. 4B, each reporter containing the intact NRSE sequence (left panel) exhibited less luciferase activity than those with the mutated NRSE sequences (right panel). 2004-05-01 In addition to the influence on the ANP gene, the NRSE sequence regulates other cardiac embryonic genes, such as BNP, skeletal α-actin, and Na, K – ATPase α3 subunit. Hence, the regulatory activity of both NRSE and NRSF in mammals prevents not only neural dysfunctions, but also physiological and phenotypical abnormalities in other non-neuronal regions of the body. 2007-06-08 The only likely repressor sequence was NRSE, which interacts with the silencer NRSF.

In addition to the influence on the ANP gene, the NRSE sequence regulates other cardiac embryonic genes, such as brain natriuretic peptide BNP, skeletal α-actin, and Na, K – ATPase α3 subunit. Hence, the regulatory activity of both NRSE and NRSF in mammals prevents not only neural dysfunctions but also physiological and phenotypical abnormalities in other non-neuronal regions of the body.

The silencer activity of this region is mediated solely by a repressor element 1 or neuron-restrictive silencer element (RE1/NRSE). Moreover, several proteins, including RE1-silencing transcription factor or neuron-restrictive silencer factor, are recruited by this regulatory sequence.

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